【摘要】 以燕麦3个品种(系)幼胚、幼穗为材料,通过组织培养方法,研究了基因型、培养基、外植体对愈伤组织诱导、继代和分化再生的影响。结果表明,对愈伤组织的诱导,基因型和培养基起重要作用,对幼胚作用极显著,对幼穗作用显著;外植体不同也影响愈伤组织形成,随培养基成分改变而变化,且幼穗较幼胚更易培养;2,4-D浓度影响愈伤组织生长和胚性愈伤组织形成,3 mg/L 2,4-D有利于愈伤生长,促进胚性愈伤形成;草莜一号幼穗愈伤组织有很强的继代能力,继代培养330 d仍具有46.58%的分化率,该材料在组织培养和基因工程研究中具有很大潜力。更多还原

【Abstract】 The influence of genotypes,medium and explant on induction,subculture and regeneration of oat callus have been studied by tissue culture of immature embryo and inflorescence of three oat varieties(line).The results showed that genetype and medium had an important role in callus induction.They were very significant and significant respectively on immature embryo and inflorescence.The formation of callus was also affected by explant and it was difference with the medium composition,inflorescence were easier to culture than immature embryo.2,4D concentration influenced callus growth and embryogenic callus generation and it can accelerate callus development and embryogenic callus generation in 3 mg/L. Callus from inflorescence of "Cao you no.1" had strong subculture capacity,it had 46.58% differentiation rate after 330 days subculture.This material had great potential in tissue culture and in genetic engineering.更多还原