【摘要】 以小卫星DNA YNZ22核心序列为引物,甜瓜DNA为模板,在甜瓜上研究了直接扩增小卫星DNA(DAMD)的优化反应体系,结果表明:在20μL的反应体系中,5种主要成分Taq DNA聚合酶、Mg2+、引物、模板DNA和dNTPs的最适浓度分别为1U,2.5 mmol/L,0.5 mmol/L,30 ng,5 mmol/L。在优化的DAMD-PCR反应体系下,利用该引物在28个甜瓜品种上构建了直接扩增小卫星DNA(DAMD)的指纹图谱,分析结果表明,该引物在28个甜瓜品种上共扩增出13位点,其中9位点具有多态性,多态性条带比率为69%,可一次性从28个甜瓜品种中鉴别出其中的21个,鉴别率高达75%。更多还原

【Abstract】 The optimal reaction of DAMD（Direct Amplification of Minisatellite DNA by PCR） in melon was studied with a minisatellite primer YNZ22 and melon template DNA.The results showed that the optimum concentration of five important components i.e.Taq DNA polymerase,Mg²⁺,primer,template DNA and dNTPs in 20 μL reaction system were 1U,2.5 mmol/L,0.5 mmol/L,30 ng,5 mmol/L respectively.The minisatellite DNA fingerprinting of 28 melon cultivars were established by using optimum system with primer YNZ22.The primer could produce 13 bands,and 9 bands were polymorphic bands,the percentage of polymorphic bands（PPB） was 69%.The primer could identify 21 of 28 cultivars at once,its identification rate was 75%.更多还原