【摘要】 采用SRAP标记对甘蓝型油菜品种1141B、垦C1、32B和32C的多态性进行了分析。每对引物组合产生13~36对比较清晰的扩增带,20对引物组合共产生419条扩增带,平均每对引物组合产生20.95条。20对引物组合共产生多态性带116条,每对引物组合产生3~10条,平均5.8条。每对引物组合产生的多态性带的比例为18.75%~38.89%,平均为28.23%。用24对引物组合对组合1141B×垦C1构建的F2群体各单株多态性进行了分析,F2群体多态性标记75条,平均为3.12条。用卡平方测验检测F2群体各单株标记基因型频率是否偏离了期望比例1∶2∶1(共显性标记)和3∶1(显性标记)的分离规律,结果表明,仅有1个SRAP标记偏离了1∶2∶1分离规律,仅占1.33%。试验结果表明,SRAP标记适于进行油菜品种的多态性分析和图谱构建,是一种经济、有效的分子标记。更多还原

【Abstract】 Polymorphism among 1141B,KenC₁,32B and 32C were analyzed with SRAP（sequence-related amplified polymorphism） markers.There were 13-36 clear polymorphic combination bands per primer,and the average was 5.8.About 419 amplified fragments and 116 polymorphic fragments were detected 20 SRAP primer combinations.The percentage of polymorphic fragments was 28.23%.The number of amplified fragments and polymorphic fragments per primer combination were 20.95 and 5.8,respectively.The polymorphism analyzed by F₂ individual of 1141B Ken C₁ with 24 primer combinations showed that there were 75 polymorphic bands,the average was 3.12.The χ² test for allelic genotype of all loci indicated that 1.33%of the SRAP markers distorted from the expectation 3∶1 ratio（dominance markers） and 1∶2∶1（co-dominance markers）.It seems that SRAP markers was a high efficiency marker system in the paper.It showed that SRAP were an effective,cheap and dependable marker for polymorphism analysis and linkage map construction in B.napus L.更多还原