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共生菌发酵法酶解魔芋飞粉制备支链氨基酸寡肽
Preparation of Oligo-peptides of Branch-chain Amino Acids from Konjac Fly Powder by Mixed Culture
【摘要】 分两步制备魔芋飞粉支链氨基酸寡肽:先采用开菲尔发酵法预处理魔芋飞粉蛋白得到可溶性肽,再用两步酶解法酶解可溶性肽得到支链氨基酸寡肽。用凝胶层析分离后的寡肽中支链氨基酸的含量为27.52%,达到了支链氨基酸平衡溶液的最适含量(25%~65%)。
【Abstract】 In order to get oligo-peptides of branch-chain amino acids from konjac fly powder,we can get peptides from proteins of konjac fly powder by mixed culture,then get oligo-peptides of branch-chain amino acids from the peptides by two process enzymatic hydrolysis.The oligo-peptides can be separated by gelatin and analyzed by automatic analytical balance.The content of branch-chain amino acids in oligo-peptides is 27.52%,just according with the most favorable condition(25%~65%).
【关键词】 魔芋飞粉;
支链氨基酸;
寡肽;
酶解;
【Key words】 konjac fly powder; branch-chain amino acids; oligo-peptides; enzymatic hydrolysis;
【Key words】 konjac fly powder; branch-chain amino acids; oligo-peptides; enzymatic hydrolysis;
- 【文献出处】 化学与生物工程 ,Chemistry & Bioengineering , 编辑部邮箱 ,2006年06期
- 【分类号】TQ464.8
- 【被引频次】10
- 【下载频次】153