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RP-HPLC测定加替沙星在家兔血中含量及药动学研究
Pharmacokinetics study of Gatifloxacin in Rabbit by RP-HPLC
【摘要】 目的探讨加替沙星在家兔体内的药动学特性。方法采用反相高效液相色谱内标法,色谱柱:ORBAX Extend-C18柱,流动相:0.01 m ol.L-1磷酸二氢钾缓冲液-乙腈(18∶5),流速1.0mL.m in-1,室温25℃。紫外检测波长为292nm,内标物为诺氟沙星。分析耳缘静脉推注加替沙星(10和20m g.k-g 1)后家兔体内的血药浓度,用药物和统计软件(DAS ver1.0)进行房室模型拟合并计算药动学参数。结果加替沙星在0.025~4μg.mL-1内线性关系良好(r=0.9973),在家兔体内药-时曲线符合一室模型。T1/2为1.25h。结论本方法操作简便,结果准确,能满足加替沙星药动学分析。
【Abstract】 OBJECTIVE To study the pharmacokinetics of gatifloxacin in rabbit.METHODS The plasma concentrations of gatifloxacin were determined by RP-HPLC method.The detection wavelength was 292nm,and norfloxacin was used as the internal standard.Data were disposed with DAS(Ver1.0 software.RESULTS The concentration-time curve of gatifloxacin was fitted to on-compartment model.T1/2 was 1.25h.CONCLUSION The study can provide pharmacokinetics parameters for rabbit.This method is simple and sensitive.
【Key words】 RP-HPLC; Gatifloxacin; Rabbit Blood; Pharmacokin etics; Determination;
- 【文献出处】 海峡药学 ,Strait Pharmaceutical Journal , 编辑部邮箱 ,2006年02期
- 【分类号】R96
- 【下载频次】175