【摘要】 目的建立HPLC测定灯盏花素脂质体中药物含量的定量方法。方法色谱柱:D IKM A D iam onsil(TM)C185μm 4.6×250 mm,流动相:甲醇-四氢呋喃-0.1%磷酸(14∶14∶72),检测波长:335nm;柱温:40℃。结果在此色谱条件下灯盏乙素与辅料及溶剂峰均得到良好的分离,灯盏乙素在5~100μg.mL-1范围内线性良好(r=0.9999),平均加样回收率为102.1%,RSD为1.26%。结论该方法简便准确,专属性强,能满足该制剂质量标准的要求。更多还原

【Abstract】 OBJECTIVE To establish an RP-HPLC method to determine the drug substance content of Breviscapine Liposomes.METHODS Using methanol-tetrahydrofuran-0.1% phosphoric acid（14∶14∶72） as mobile phase,the separation was performed on a DIKMA Diamonsil（TM） C₁₈（5μm 4.6×250nm） column with a flow rate of 1.0mL·min^-1.The detective wavelength was 335nm and the column temperature was 40℃.RESULTS Peaks for scutellarin,excipients or solvents were separated well in the chromatographic file with the RP-HPLC method.Regression analysis of the data of scutellarin concentration against its peak area showed good straight line over the range:5～100 μg·mL^-1（r=0.9999）.And the average recovery was 102.1%.CONCLUSION The method was simple,specific and accurate,and can effectively control quality of Dangguisini granules.更多还原