【摘要】 目的探讨核转录因子(nuclearfactorofkappaB,NF-κB)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)在人胃癌细胞中表达及其与临床病理特征的相关性。方法利用荧光定量聚合酶链反应法测定32例人胃癌细胞和25例癌旁细胞内NF-κBmRNA和VEGFmRNA的定量表达,分析其与临床病理特征的相关性。结果人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达与性别、年龄无关(P>0.05)。人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达明显高于癌旁细胞的表达(P<0.01)。人胃癌细胞中NF-κBmRNA和VEGFmRNA的表达与组织类别、浸润深度、分化程度、淋巴结转移、分期和大小有关(P<0.01)。人胃癌细胞中NF-κBmRNA与VEGFmRNA呈无显著相关性(r=0.2196,P=0.2271)。结论NF-κBmRNA表达上调可能与人胃癌发生、发展和转移有关。更多还原

【Abstract】 Objective to determine expression of nuclear factor of kappa B and vascular endothelial growth factor, and its relation to clinicopathological characteristics in human gastric cancer. Methods Expression of NF-κB mRNA andVEGF mRNA in 32 cases gastric cancer and their adjacent noncancerous tissues was measured by fluorescent quantitative polymerase chain reaction(FQ-PCR). Results Expression of NF-κB mRNA and VEGF mRNA was not correlated with sex, age in human gastric cancer(P>0.05). Expression of NF-κB mRNA and VEGF mRNA in human gastric cancer was significantly higher than that in their adjacent noncancerous tissues(P<0.01). Expression of NF-κB mRNA and VEGF mRNA was correlated with histological grades, depth of tumor invasion, tumor differentiation, lymph node metastasis and tumor size in human gastric cancer(P<0.01). No significant correlation in human gastric cancer were found between expression of NF-κB mRNA and VEGF mRNA(r=0.2196, P=0.2271). Conclusions Our results show that overexpression of NF-κB mRNA and VEGF mRNA could play an important role in the development, process and metastasis of human gastric cancer.更多还原