【摘要】 目的初步探讨卡介苗(Bacillus Calmette-Guerin,BCG)分泌性蛋白在体外培养试验中对人单个核细胞的刺激效应。方法用淋巴细胞转化试验(MTT法)检测单个核细胞经卡介苗纯蛋白衍化物(BCG-PPD)刺激后的增殖程度和IL-2生成情况,同时设ConA对照组。结果BCG-PPD体外刺激人单个核细胞后,单个核细胞增殖程度和IL-2生成量均明显增加,且显著高于有丝分裂原Co-nA对照组(P均<0.001);单个核细胞增殖程度和IL-2生成量之间呈正相关关系(r=0.6 666,P<0.001)。结论BCG分泌性蛋白具有较强的免疫原性,可显著激活单个核细胞功能,提示卡介苗分泌性蛋白在抗结核免疫中起了积极作用。更多还原

【Abstract】 Objective To observe the influence of Bacillus Calmette-Guerin(BCG)’s secretory protein to human mononuclear cells(MCs). Methods The proliferation and IL-2 production of human MCs stimulated with BCG’s purified protein derivative(BCG-PPD) were detected by lymphocyte transformation test(LTT) and enzyme-linked immunosorbent assay(ELISA).Results After stimulated with BCG-PPD in vitro,the proliferation and IL-2 production of human MCs were remarkably higher than ConA control group(P<0.001).Significant correlation was showed between the proliferation degree and IL-2 production of human MCs(r=0.6 666,P<0.001). Conclusion BCG’s secretory protein has strong immunogenicity can activate human MCs.更多还原