【摘要】 目的探讨白细胞介素7(IL-7)、白细胞介素2(IL-2)协同对肺结核病患者外周血单个核细胞(PBMC)分泌干扰素γ(IFN-γ)、白细胞介素4(IL-4)的影响。方法随机将20例肺结核病患者和15例健康对照者PBMC按加入刺激物的不同分为RPMI-1640组、PPD组、PPD+IL-7组、PPD +IL-2组,PPD+IL-7+IL-2组,培养72小时后收集上清液,采用酶联免疫吸附法(ELISA)检测各组培养上清液中IFN-γ、IL-4的水平。结果与PPD组相比,加入IL-7或IL-2均能有效促进肺结核患者及健康对照者PBMC分泌IFN-γ(P<0.05),IL-7可抑制IL-4的合成(P<0．05);同时加入IL-7和 IL-2可显著增加肺结核病患者及健康对照者PBMC分泌IFN-γ(P<0．01),抑制IL-4的合成(P< 0．05)。结论IL-7和IL-2协同可显著促进IFN-γ分泌,抑制IL-4合成,从而调节Th1／Th2平衡,对结核分枝杆菌感染患者发挥保护性的免疫反应。更多还原

【Abstract】 Objective To investigate the synergistic effects of IL-7 and IL-2 on the production of in-terferon-γ(IFN-γ) and IL-4 by peripheral blood mononuclear cells(PBMC) from patients with tuberculosis. Methods PBMCs were isolated and cultured from 20 patients with pulmonary tuberculosis and 15 healthy tuberculin reactors respectively. According to the different stimulators, every sample was divided into five groups: RPMI1640, PPD, PPD+IL-7, PPD+IL-2, PPD + IL-7 + IL-2. After 72 hours, the supernatants of culture PBMC were collected. The levels of IFN-γ and IL-4 were measured by ELISA. Results Compared with the PPD group, IL-7 or IL-2 was increased the production of IFN-γ(P<0. 05), IL-7 Could decreased the production of IL-4 in the patients and the healthy individuals(P <0. 05), IL-7 and IL-2 could increased significantly the production of IFN-γ(P<0. 01), but decreased the production of IL-4(P<0. 05). Conclusion IL-7 and IL-2 could regulate the balance between Th1 and Th2 cytokines by significantly inducing IFN-γ production and inhibiting IL-14 expression, which suggested IL-7 and IL-2 enhance the patient defense against the infection of tuberculosis.更多还原