【摘要】 目的探讨桂皮酸对K562细胞凋亡的诱导作用及其机制。方法应用MTT测出 IC50,应用免疫荧光显微镜,DNA Ladder,流式细胞仪,检测凋亡的发生,应用RT-PCR琼脂糖凝胶电泳检测bcl-2基因表达的差异。结果经MTT检测发现IC50为1 mmol／L,24 h后药物基本达到了较佳的效果,且免疫荧光显微镜、DNA Ladder、流式细胞仪检测均有凋亡的发生,RT-PCR结果显示,经桂皮酸处理24h后,bcl-2基因表达明显降低。结论1 mmol／L的桂皮酸作用24 h后可以引起K562 细胞发生凋亡,并且bcl-2基因表达下调是引起凋亡的原因之一。更多还原

【Abstract】 Objective To investigate K562 apoptosis induction by cinnamic acid as well as the related action of bcl-2 gene. Methods Divide K562 cells into 2 groups: the cinnamic acid was added to group A, group B was as control group. The K562 cells in two groups were tested by MTT, fluorescence microscopy, flow cytometry, RT-PCR, electrophoresis. Results poptosis was detected in group A by fluorescence microscopy and electrophoresis respectively. The expression of bcl-2 was statistically significant between two groups tested by RT-PCR, B>A (P<0. 05). Conclusion The results of this investigation showed cinnamic acid may induce the apoptosis of K562 cells by downregulation of bcl-2 expression.更多还原