【摘要】 目的:探讨血小板衍生生长因子-BB(PDGF-BB)对人牙周膜(PDL)细胞在离体牙根面上附着、增殖的影响。方法:应用细胞计数法和扫描电镜观察法,观察在细胞因子作用下,离体正常和牙周病患牙根面上PDL细胞附着和增殖的情况。结果:无论是否加入PDGF-BB,PDL细胞在正常牙根面(N组)上的附着数量均明显多于病变(P组)根面(P<0.01);与对照(C组)相比,PDGF-BB(S组)既能促进PDL细胞在正常根面的附着(P<0.01),也能促进PDL细胞在牙周病变根面上的附着(P<0.05)。不加PDGF-BB时,PDL细胞在NC上的增殖率为80.0%,PC只有50%;加入PDGF-BB后,NS增殖率为249.4%,PS的增殖率为213.09%,与对照(C)组相比,PDGF-BB既能明显促进PDL细胞在正常根面的增殖(P<0.01),又能显著提高PDL细胞在病变牙根面上的增殖(P<0.01);扫描电镜下病变牙根面上生长的PDL细胞数量较少,形态细长,细胞的突起伸展不够充分,边缘皱缩,与根面附着较松散,经PDGF-BB作用后,细胞与根面的附着状态有所改善,较为紧密,数量有所增加。结论:PDGF-BB是一种有效的促PDL细胞生长的因子,可促进PDL细胞在牙根面的附着和增殖。更多还原

【Abstract】 Objective:To evaluate the effects of platelet-derived growth factor-BB(PDGF-BB) on the attachment and proliferation of periodontal ligament(PDL) cells on different root surfaces.Methods: The attachment and proliferation of PDL cells on root surfaces of extracted teeth with or without periodontal disease was studied using cell culture and scanning electronic microscopy.Results: The PDL cells attached more on root surface of normal teeth(Group N) than on affected teeth(Group P) either with or without addition of PDGF-BB(P<0.05).Compared with controls(Group C),PDGF-BB(Group S) stimulated the attachment of PDL cells both on normal root surface(P<0.01) and affected root surface(P<0.05).The rates of PDL cell proliferation were 80% and 50% respectively on groups NC and PC without PDGF-BB,and when PDGF-BB was added,rates of PDL cell proliferation in groups NS and PS were 249.4% and 213.09%.Compared with controls(Group C),PDGF-BB significantly stimulated the proliferation of PDL cells both on normal root surface(P<0.01) and affected root surface (P<0.01).As shown by scanning electronic microscopy,PDL cells on affected root surface were less in quantity,slender in shape,with inadequate extension of cellular dendrites and shrinkage of cell borders.Stimulation with PDGF-BB markedly improved the cell attachment to the root surface and numbers of the cells.Conclusions: PDGF-BB appeared to be a positive growth factor to promote the attachment and proliferation of PDL cells.更多还原