【摘要】 将λ-DNA与荧光染料YOYO-1结合,利用荧光显微技术对DNA分子在毛细现象作用下进入宽40 nm、深60 nm的纳米沟道内,并在其内部被拉伸以及沿沟道移动的情形进行了观察.讨论了DNA分子在沟道内的运动情况.结合光学分子成像技术与该尺寸范围的纳流芯片,将有助于研究生物分子的动力学和静力学性质.更多还原

【Abstract】 λ-phage DNA molecules, stained with YOYO-1 fluorescent dye, were put inside sub-100 nm open channels which have width ~40 nm and depth ~60 nm. The DNA molecules were transported along these nanoconduits and the removing information was observed by fluorescence microscopy technique clearly. The movements of DNA molecules in the channels were discussed. Associating with optical molecular imaging, these scale nanostructured fluidic channels would be useful for studying single biomolecules statics as well as the dynamics.更多还原