【摘要】 目的:从鲜人参中分离纯化人参蛋白。方法:运用生物化学技术,通过盐析分离后,再经过透析、离子交换、凝胶过滤等层析技术达到分离目的。结果:分离纯化出一种人参蛋白,经过HPLC鉴定其纯度为90%以上,经SDS-聚丙烯酰胺凝胶电泳检测其分子量为20.1KD到31.0KD之间。结论:通过本文所述方法可以得到纯度较高的特种人参蛋白,为将来进一步对人参蛋白活性及结构研究奠定了基础。更多还原

【Abstract】 Objective: To purify ginseng protein from fresh ginseng. Methods: Biological technique,including salting out,dialysis,ion-exchange chromatography and gel filtration,was used. Results: One kind of ginseng protein was obtained with high purity than 90% analyzed by HPLC and with molecular weight between 20.1KD and 31.0KD obtained from SDS-PAGE. Conclusion: Ginseng protein of higher purification could be obtained through the above-mentioned methods. The research on its activities and structure may be developed on the basis of these results.更多还原