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仙人掌多糖提取过程中三种脱蛋白方法的比较研究

Comparison of three Methods of Removing Protein from Polysaccharide Extract in the Cactus

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【作者】 史德芳杨洋孙晓雪任红韩淑琴

【Author】 Shi De-fang1,Yang Yang2,Sun Xiao-xue1,Ren Hong1,Han Shu-qin1 (1.College of Light Industry and Food Engineering,2.College of Life Science,Guangxi University,Nanning 530004)

【机构】 广西大学轻工与食品工程学院广西大学生命科学学院广西大学轻工与食品工程学院 广西南宁530004教授

【摘要】 用水提取的仙人掌多糖常含有蛋白质,实验以多糖损失率和脱蛋白率为衡量指标,选用了3种方法:Sevag法、三氯乙酸法和酶法对仙人掌多糖提取物进行脱蛋白处理。实验结果表明:酶法、三氯乙酸法和Sevag法的脱蛋白率分别为89.6%、56.2%和45.5%,多糖损失率分别为7.2%、9.5%和13.6%。经比较认为:酶提取法是替代常规方法的一种有效方法。

【Abstract】 In order to obtain the purified polysaccharide from cactus,the effects of three methods for removing protein from the extracted polysaccharide from cactus was studied by comparing the percentage of the decomposed polysaccharide (PDP) and the removed protein (PRP). The results showed that the PRP for the method of using enzyme、using CCl3COOH and using Sevag were listed as follows: 89.6%、56.2% and 45.5%,whilie the PDP for three methods were 7.2%、9.5% and 13.6%,respectively. Compared with normal method,the application of enzymatic removal of protein was an effective and alternative method.

【关键词】 仙人掌多糖脱蛋白
【Key words】 CactusPolysaccharideRemoval of proteinEnzyme
  • 【文献出处】 现代食品科技 ,Modern Food Science and Technology , 编辑部邮箱 ,2006年04期
  • 【分类号】TS201
  • 【被引频次】42
  • 【下载频次】501
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