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一株琼胶酶高产菌株的筛选鉴定及产酶条件的优化
Isolation and Identification of an Agarase-Producing Marine Gram Positive Bacteria
【摘要】 本研究从海水中分离筛选到一株琼胶酶高产菌,编号是2.2-g,革兰氏染色为阳性,呈细杆状。通过DNS法测定,其分泌琼胶酶的酶活为0.066U。通过16SrDNA的PCR扩增、序列测定,测序结果(AY949021)与Genebank数据库进行比较,发现它与Brevibacteriumaureum(AY299093)和Brevibacteriumlinens(AY243345)同源性高达99%,因此可以判断其为短杆菌(Brevibacteriumsp)。
【Abstract】 A straight Gram-positive bacteria containing highyield agarase was selected from sea water and named 2.2-g. The enzyme activity of agarase isolated from the bacteria was 0.066 U detected by DNS method. By means of 16S RNA analysis and compared the analysis results with Genebank database,it had 99% homology to Brevibacterium aureum (AY299093),and Brevibacterium linens (AY243345). It belonged to Brevibacterium sp.
- 【文献出处】 现代食品科技 ,Modern Food Science and Technology , 编辑部邮箱 ,2006年04期
- 【分类号】TS201
- 【被引频次】19
- 【下载频次】353