【摘要】 目的探讨银杏叶提取物(EGb761)对大鼠局灶性脑缺血再灌注后Caspase-3、Caspase-9活性的影响。方法2月龄Wistar大鼠随机分为假手术组(组A)、缺血组(组B)、小剂量EGb组(组C)、大剂量EGb组(组D)。建立大鼠大脑中动脉缺血1.5h再灌注24h的模型。C、D组于术前1h分别腹腔内注射银杏叶提取物。采用四肽酶底物法检测Caspase-9、Caspase-3活性水平;用TTC法检测梗死体积。结果(1)Caspase-9、Caspase-3活性A组未检测到Caspase-9、Caspase-3活性升高,B组(再灌注24h)Caspase-9、Caspase-3活性明显高于C、D组,且C组高于D组;(2)梗死体积A组脑组织无损伤,再灌注24hB组脑梗死体积明显高于C、D组,且C组高于D组。结论EGb761能够减轻大鼠缺血性脑损伤,实现保护作用的机制可能是通过降低Cas-pase-9、Caspase-3活性实现的,并且疗效与剂量有关。更多还原

【Abstract】 Objective To explore the influence of EGb761 on the activities of Caspase-9、Caspase-3 following focal cerebral ischemia in rats.Methods Two-month-old Wistar rats were randomly divided into controlled group(group A) ,ischemic reperfusion group(group B),low-dose EGb761 group (group C) and high-dose EGb761 group (group D),EGb761 of 50/kg and 100 mg/kg were given intraperitoneally to rats in group C and D 1 h before focal cerebral ischemia operation respectively .The activities of Caspase-9、Caspase-3 were measured by cleavage of the fluorescence substrate AC-LEHD-AFC and AC-DEVD-AMC respectively ;Brain infarction volumes were evaluated by TTC procedures.Results (1)The activities of Caspase-9、Caspase-3:They were not detected in the controlled group ,and increased at 24 h timepoint after MCAO in group B ,significantly decreased in group C and D,especially less in group C than in group D ,there was a asignificant difference among group B 、C and D. (2)The infarction volumes:Brain of rats was not injuried in the sham-operated group ,but they were more in group B than in group C and D,and morein group C than in group D.Conclusion EGb761 could ease ischemic cerebral injury of rats andone of its protective role may be realized by inhibiting the activies of Caspase-9 and Caspase-3,having a relationship between does and effect.更多还原