【摘要】 目的通过体外实验,了解氯化镉(CdCl2)对中国仓鼠肺成纤维细胞(V79细胞)的直接DNA损伤作用及锌的拮抗作用,初步探讨镉的遗传毒性机制及预防措施。方法采用甲基四唑法(MTT法)了解CdCl2对于V79细胞的毒性,通过碱性单细胞凝胶电泳常用的几个评价指标,研究CdCl2体外对于V79细胞DNA的断裂损伤,并采用生理浓度ZnCl2(10μmol/L)加以拮抗。结果MTT试验中,CdCl2对于V79细胞的毒性随染毒剂量增加而增高,呈线性关系,线性方程为:y=-0.009 4x+94.472;在碱性单细胞凝胶电泳中,CdCl2单独染毒,随着染毒剂量增加,DNA损伤加重,而锌具有明显的拮抗作用。结论本实验条件下,CdCl2能造成V79细胞DNA损伤,生理浓度的锌可以拮抗镉的DNA损伤效应。更多还原

【Abstract】 Objective To investigate the effects of Cd²⁺ and Zn²⁺ on DNA damage of Chinese hamster lung fibroblast cells（V79）in culture.Methods Methyl tetrazolium test（MTT）assay was used to detect the cytotoxicity of CdCl₂ on V79 cell line.By general indexes of SCGE assay,DNA strand breaks induced by CdCl₂ in vitro on V79 cells and with zinc as antagonizer were observed.Results In MTT test,the cytotoxicity effect of CdCl₂ on V79 raised as the increase in dosage showing linear relationship with the linear equation y=-0.009 4 x+94.472.In alkali SCGE assay,CdCl₂ could damage the DNA of V79 proportionally with the dosages.ZnCl₂ showed obvious antagonistic effect.Conclusions CdCl₂ could induce DNA damage on V79 cells under the experimental condition,while Zn in physiological concentration may antagonize the damage.更多还原