【摘要】 目的探讨丙烯腈(ACN)对雄性SD大鼠睾丸组织的病理学损伤作用及超微结构的改变。方法从52只雄性SD大鼠中随机取出4只作为空白对照组(不做任何处理),其余48只随机分为4组,每组12只,分别以0,7.5,15.0,30.0 mg/kg的剂量(用生理盐水作溶剂)腹腔注射ACN染毒,1次/d,每周5次,连续染毒13周。13周末,从各组中随机取6只大鼠处死;其余实验大鼠停止染毒2周后处死。用光镜和电镜观察大鼠睾丸组织的病理损伤及超微结构的改变。结果ACN染毒13周后,7.5 mg/kg组光镜下可见大鼠睾丸曲精小管组织病理学结构有轻微改变,受损程度随染毒剂量的增加而加重;30.0 mg/kg组,光镜下可见大鼠睾丸部分曲精小管出现退行性病变,管腔中生精细胞和精子数量减少;电镜下可看到凋亡的、核畸形的以及坏死的初级精母细胞和畸形精子,生精管上皮空泡样变,腔面无精子,尚存的发育期精子细胞内没有线粒体的相应移动。15周后(停止染毒2周后)各组大鼠睾丸组织病变与同剂量组染毒13周末比较,差异无显著性。结论ACN能够诱导大鼠睾丸损伤,具有性腺毒性,同时提示,生精细胞的核质损伤是其主要毒作用之一。更多还原

【Abstract】 Objective To explore the effects of acrylonitrile（ACN）on histopathology and ultra-microstructure of testis in rats.Methods Acrylonitrile was administered intraperitoneally to male Spraque-Dawley（SD）rats once a day,5 times a week for 13 weeks at the doses of 0,7.5,15.0 or 30.0 mg/kg body weight,respectively.Random samples of six rats from each group were sacrificed at the end of the 13^th week,so were done the rest at the end of the 15^th week.Changes of histopathology and ultra-microstructure in testis of rats were observed with light microscope and electron microscope.Results Under light microscope,slight changes were found in small seminiferous tubule of testis in rats in 7.5 mg/kg body weight group.Degree of damage to testis of the rats was increased with dose after ACN administration for 13 weeks.In 30 mg/kg body weight group,degeneration of part of seminiferous tubule,decrease in the number of spermatogenic cells and spermatozoa in the tubules were observed,germs with nucleus shrink in part of the tubules and slight edema in interstitial were noted.Under electron microscope,primary spermatocytes in apoptosis or necrosis and primary spermatocytes with abnormal nucleus and spermatozoa with abnormal head were found in 7.5 mg/kg body weight group.No corresponding movement of mitochondria in developing spermatid and seminiferous epithelial vacuolar degeneration was found in 30.0 mg/kg body weigh group.There was no significant difference in pathologic injury to the testes of the rats sacrificed between the end of the 13^th week and the end of the 15^thweek at same dose.Conclusions These results indicate that ACN could induce injury to testis and produce gonadal toxicity in male rats,which mainly due to the nuclear injury of spermatogenic cells during spermatogenesis.更多还原