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L-鸟氨酸的混合菌种发酵研究

Studies on multi-bacterium fermentation of L-ornithine

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【作者】 李环周磊陈悦韦萍欧阳平凯

【Author】 LI Huan,ZHOU Lei,CHEN Yue,WEI Ping,OUYANG Pin-kai(College of Life Science and Pharmacy,Nanjing University of Technology,Nanjing 210009)

【机构】 南京工业大学制药与生命科学学院南京工业大学制药与生命科学学院 南京210009南京210009

【摘要】 谷氨酸棒杆菌可发酵葡萄糖产L-鸟氨酸。通过连续筛选与驯化筛选到了产量较高的生产菌株CG1006。以GC/MS对发酵液成分的定性分析及鸟氨酸的产量为依据,优化了培养条件,产量从最初的14.346g/L提高到40.416g/L。初步考察了酵母菌CG1006-1对发酵液成分及产量的影响。结果表明混合菌种发酵可纯化发酵液成分。

【Abstract】 L-ornithine can be produced from glucose by Corynebacterium Glutamicum. A high yield strain CG1006 was obtained by many times of selection and cultivation.On the bases of the qualitative analysis of fermentation broth by GC-MS and the quantitative analysis by HPLC,the culture medium was optimized.And the yield was improved from(14.346)g/L to 40.416 g/L.The effects of yeast 1006-1 on the L-ornithine yield and the fermentation component were(investigated.) A new idea of coupling technology between fermentation and purification was put forward.

  • 【文献出处】 工业微生物 ,Industrial Microbiology , 编辑部邮箱 ,2006年04期
  • 【分类号】TQ922.9
  • 【被引频次】9
  • 【下载频次】362
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