【摘要】 研究了E.coliK-12转醛醇酶基因(talB)在自身启动子和在Z.mobilisCP4eno基因启动子的启动下在E.coliDH5α和Z.mobilisCP4中的表达情况。首先克隆了E.coliK-12talB基因,并连接到穿梭载体pZB1上构建成pZB1-talB;然后利用PCR重叠延伸技术将E.coliK-12talB自身的启动子换成Z.mobilisCP4eno的启动子,构建得到pZB1-Peno-talB。将这两个质粒分别转化E.coliDH5α和Z.mobilisCP4。对转化子粗酶液进行的转醛醇酶酶活力测定结果表明,E.coli talB自身启动子和Z.mobilis eno启动子能以基本相同的效率启动talB基因在E.coli和Z.mobilis中的表达。更多还原

【Abstract】 The expression of E.coli K-12 talB gene in Z.mobilis CP4 under the control of its own promoter and the Z.mobilis CP4 eno promoter was studied.First,talB gene from E.coli K-12 was cloned and inserted into a shuttle vector pZB1 to form plasmid pZB1-talB.Second,the talB native promoter was replaced by the eno promoter from CP4 using PCR overlap extension method,and also introduced into pZB1 to form plasmid pZB1-Peno-talB.Then,both of the recombinant plasmids,pZB1-talB and pZB1-Peno-talB,were transformed into E.coli DH5α and Z.mobilis CP4.The transaldolase activity determinations showed that talB and Peno-talB expressed in E.coli and Z.mobilis with equal efficiency.更多还原