【摘要】 应用Plackett-Burman设计试验从L-亮氨酸基础发酵培养基的10种成分中筛选出5种重要成分,然应用响应面分析试验确定5种重要成分的最适用量。培养基优化后摇瓶分批发酵72h产L-亮氨酸18.05g/L更多还原

【Abstract】 Five main factors were screened by the Placket-Burman design method from ten ingredients of L-leucine basic fermentation medium. Then,the best level of five factors was determined by response surface analysis. The strain TQ9806-1 could produce L-leucine 18.05 g/L after fermentation with optimum medium for 72 hours by flask-shaking batch fermentation.更多还原