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转PSAG12-ipt基因水稻叶片中叶绿体超微结构的变化

Ultrastructure changes of chloroplast in leaves of transgenic rice (PSAG12-ipt)

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【作者】 王亚琴夏快飞

【Author】 WANG Ya-qin1, XIA Kuai-fei2 ( 1.College of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, China; 2.School of Life Sciences, Sun Yat-sen University,Guangzhou 510275, China )

【机构】 华南理工大学生物科学与工程学院中山大学生命科学学院 广东广州510640广东广州510275

【摘要】 研究了转PSAG12-ipt基因水稻和对照植株发育过程中叶片中的叶绿体结构的变化。发现水稻发育到乳熟期,转基因植株叶片中的叶绿体与对照植株开始出现明显的差别。对照叶绿体中嗜锇体体积增大,数目增多,大部分基粒的类囊体膜膨胀、裂解,片层结构解体。而转基因植株叶片中的叶绿体结构变化不大,嗜锇体相对有所增加,但体积较小,大部分基粒类囊体片层结构仍然排列整齐,少数类囊体垛叠化丧失。

【Abstract】 The structure of chloroplast was investigated during its development in transgenic rice(PSAG12-ipt)and control. The results revealed that there was an obvious differentiation of chloroplasts between transgenic rice and control during milky ripe stage. In control,the size and number of osmiophilic granules increased greatly and the thylakoid membrane of most grana swelled and split and most grana lamellae disappeared. Whereas in transgenic rice,only the number of osmiophilic granules increased and most grana-thylakoid lamellae arranged in order.

【关键词】 PSAG12-ipt基因叶绿体超微结构
【Key words】 PSAG12-ipt genechloroplastultrastructure
【基金】 广东省国际合作项目(2004B50201021)~~
  • 【分类号】S511
  • 【被引频次】4
  • 【下载频次】180
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