【摘要】 目的:探讨己酮可可碱(PTX)对脂多糖(LPS)诱导的内皮细胞单层通透性的保护作用。方法:建立内皮细胞单层损伤模型,随机分为LPS组、PTX组、LPS+PTX组、对照组,测定内皮单层滤过系数、蛋白质渗透压反射系数及进行内皮细胞单层形态学检查。结果:LPS组内皮单层滤过系数显著升高、蛋白质渗透压反射系数显著降低;与正常对照组比较,PTX组的滤过系数和蛋白质渗透压反射系数无明显变化;与LPS组比较,LPS+PTX组两系数及内皮细胞单层形态学检查无明显变化,近似于正常对照组。结论:PTX无降低正常内皮细胞单层通透性的作用;PTX对LPS诱导的内皮细胞单层通透性有保护作用,其机制可能与PTX拮抗LPS导致内皮细胞单层通透性增加的某些环节有关。更多还原

【Abstract】 Objective:To study the effects of pentoxifylline(PTX) on LPS-induced endothel cell monolayer permeability.Methods:To observe the influence of PTX on the permeability of in vitro cultured endothelial cell monolayer(ECM) in LPS-induced ECM injury model with human umbilical vein endothelial cells(HUVEC) in LPS group,PTX group,LPS+ PTX group and control group by determining the changes of the filtration coefficients(Kf) and the osmotic reflection coefficients(σ),and the histological appearance of endothelial cell monolayer under microscope.Result:PTX in LPS+ PTX group could markedly decrease the Kf of ECM,enhance the osmotic reflection coefficients of that compared with those in LPS group.Conclusion:PTX could protect LPS-induced endothel cell monolayer permeability.更多还原