【摘要】 三维显微图像去卷积计算量巨大,运算时间长。评价和分析了三维点扩散函数、散焦像、光学切片以及不同三维点扩散函数去卷积的复原图像,进一步证明了散焦光信息对焦面像的干扰,主要来自于焦面像两侧附近的散焦像。提出了折中选择三维PSF的空间大小,可以获得良好的复原效果,并且减少运行时间。提出了频谱图均值的新概念,作为评价图像清晰及模糊程度的标准,并运用于散焦像、光学切片以及复原结果图的评价。更多还原

【Abstract】 The deconvolution for 3D image of wide-field microscope need to process very large data size,and it spend a lot of time.3D PSF(three dimensional point spread function),out-of-focus images,optical section images and restored images deconvolved by different 3D PSF were estimated and analyzed.It was proved further that the disturbance of out-of-focus light to in-focus images was from out-of-focus images of both sides.It was proposed that 3D microscopy deconvolution could get more perfect effect of image restoration and the runtime could be spend less through selecting appropriate size of 3D PSF.And a new standard for estimating the definition or fuzzy degree of image was proposed,and it was applied to estimation of out-of-focus images,optical section images and restored images.更多还原