【摘要】 毛竹叶总黄酮可以用作食品抗氧化剂、药剂、化妆品添加剂等,有着广阔的市场前景。今用30%的乙醇溶液(60℃,固液比1:10,浸取时间为1h)从毛竹叶中浸取得总黄酮粗提物,再用液-液萃取法从粗提物中分离总黄酮;重点考察了原料液浓度、萃取剂用量、萃取次数等对萃取过程的影响,建立了一条从毛竹叶中提取总黄酮的工艺路线。萃取过程的合适工艺条件为:30℃下,选用水饱和的正丁醇作为萃取剂,粗提物原料液浓度在0.733~0.956g·L-1,相比R=Vorg/Vaq=1,萃取三次。此时总黄酮的萃取率达到72.3%,总黄酮含量由2.3%(粗提物)提高到8.3%。更多还原

【Abstract】 The total flavonoids extracted from leaves of Phyllostachys pubescens have multiple biological effects, such as anti-free radical, anti-oxidation, anti-aging, anti-fatigue anti-bacteria, anti-virus and prevention of cardiovascular diseases, and they can be used as a pharmaceutical intermediate, dietary supplement, cosmetic ingredient and food antioxidant. In this study, a process for extracting and separating total flavonoids from leaves of Phyllostachys pubescens was presented. First, the solution of ethanol-water (3:7, v/v) was used to extract total flavonoids from the leaves (temperature 60℃; mass of leaves to volume of solvent 1:10, g/mL; extraction time 1 h). Then the crude sample was purified by solvent extraction. In order to optimize the extraction process, the suitable extractant was chosen and the influences of feed concentration, solvent load and extraction times on the extraction yield and purity were investigated, respectively. It turns out that, under the following conditions: temperature of 30℃, extractant of water-saturated n-butanol, feed concentration of 0.733~0.956 gL1, R (ratio of solvent volume to feed volume) of 1 and extraction times of three, the yield reaches 72.3%, and the purity is raised from 2.3 % (crude sample) to 8.3 %.更多还原