【摘要】 应用单因素试验法研究发酵培养基pH值、发酵温度、摇瓶装液量、摇瓶转速等发酵培养条件对L-亮氨酸发酵的影响。试验结果显示,500 mL三角瓶装液量50 mL,置旋转摇床上转速220 r/m in,pH 7.0、培养温度28℃,发酵72 h,产L-亮氨酸18.54 g/L。更多还原

【Abstract】 The effect of culture conditions such as pH value in fermentation medium,temperature,capacity in shaking-flask,shaking speed on L-leucine fermentation were studied by means of single factor experiment.It shows that when pH value in fermentation medium is 7.0,50mL medium in each 500mL flask after fermented for 72 hours at the temperature of 28℃ the production of L-leucine reaches 18.54g/L on a 220r/min shaking speed.更多还原