【摘要】 目的:为控制新药更舒安颗粒的产品质量,建立其有效成分淫羊藿苷的定量方法。方法:淫羊藿苷的定量测定采用RP-HPLC法。色谱柱为ZORBAXSBC18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70),流速1.00mL/min;柱温为25℃;检测波长为270nm。结果:淫羊藿苷在测定条件下,平均回收率为101.18%,RSD=0.62%,回归方程为Y=20.121X-3.7077,r=1.0000;最低检出限度为0.12μg/mL,线性范围在1.22-24.48μg/mL;精密度试验淫羊藿苷峰面积RSD为0.58%。结论:本方法准确、简便、专属性强、灵敏度高,结果稳定,适用于更舒安颗粒的质量控制。更多还原

【Abstract】 Objective To establish RP-HPLC method for the determination of icariin in Gengshu’anGranules. Methods The Column used was ZORBAX SB C18(250 mm×4.6 mm, 5μm), the mobile phaseused was acetonitrile-water (30∶70), the flow rate was 1.00 mL/min with the detection wavelength at270 nm. Results The minimum detection limit is 0.12μg/mL, the average recovery was 101.18% withRSD 0.62%, the calibration curve was the linear in the range of 1.22—24.48 μg/mL, r=1.000 0.Conclusion The method is accurate, simple, specific, sensitive, and result is stable. It can be used forthe quality control of Gengshu’an granules.更多还原