【摘要】 为建立一种简便、准确、实用的人肿瘤坏死因子A-308基因突变频率的检测方法,了解广东汉族人肿瘤坏死因子A-308基因的分布特点,应用聚合酶链反应(PCR)特异性扩增正常人肿瘤坏死因子A-308基因序列,扩增产物用限制性内切酶NcoⅠ酶切,琼脂糖凝胶电泳后,观察酶切位点的限制性片段长度多态性(RFLP)图谱,并结合文献进行了不同种族间的分析比较。结果表明,230例广东籍汉族人中,TNF-α1/1、TNF-α1/2、TNF-α2/2表型频率分别为:0.886 9、0.108 7、0.004 4,TNF-α1、TNF-α2基因频率分别为:0.941 3、0.058 7。该法简便、快速、准确,适合于一般实验室检测及大规模的人群调查,肿瘤坏死因子A-308基因多态性在不同种族间分布存在着明显的差异。更多还原

【Abstract】 To establish a simple,accurate and practical method for TNF-308 genotyping and identify the distribution feature of TNF-α-308 G/A gene in the Hans in Chinese,TNF-α G/A gene sequences were amplified by using polymerase chain reaction(PCR).The PCR products were digested with Nco Ⅰ and subjected to electrophoresis on agarose gels.The patterns of restriction fragment length polymorphism(RFLP) of TNF-α-308 G/A gene were distinguished.The results showed that 230 health individuals were investigated with this PCR-RFLP method.The gene frequency of TNF-α-308 was as followed.TNF-α 1/1,TNF-α 1/2,TNF-α 2/2 genotype frequency:0.886 9、0.108 7、0.004 4,TNF-α 1、TNF-α 2 Genefrequency:0.941 3,0.058 7.It concluded the PCR-RFLP method was considered a rapid and simple technique for obtaining accurate results and also suitable for routine laboratories and large-scale population studies,TNF-α-308 G/A genes distribution in the Hans in Chinese is undifferent from those in other areas.更多还原