【摘要】 目的对比分析检测HBV前S1抗原、HBV-DNA和HBeAg在乙型肝炎感染期主要模式组中的临床意义。方法用酶联免疫吸附法(ELISA)分别检测HBV前S1抗原和乙肝血清标志物“五项”,荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA,对308例检测结果进行统计分析。结果308例乙型肝炎患者血清标志物“五项”4种模式组即“HBsAg、HBeAg、HBcAb”阳性模式组、“HBsAg、HBeAb、HBcAb”阳性模式组、“HBsAg、HBeAg”阳性模式组和“HBsAg、HBcAb”阳性模式组。前S1抗原总阳性率为62.34%(192/308),HBV-DNA总阳性率为78.90%(243/308)。在HBe阳性模式组与HBeAg阴性/抗HBeAb阳性模式组对比,前S1抗原总阳性率分别为71.65%(139/194)和46.49%(53/114),阳性率差异具有统计学意义(χ2=18.30,P<0.01)。HBV-DNA阳性组和HBV-DNA阴性组前S1抗原阳性率分别为74.90%(182/243)和15.38%(10/65),阳性率差异具有统计学意义(χ2=74.85,P<0.01)。前S1抗原与HBeAg检测符合率为64.94%,与HBV-DNA检测符合率为76.95%。结论检测HBV前S1抗原阳性率在HBe阳性模式组中明显高于HBeAg阴性/抗HBeAb阳性模式组;前S1抗原与HBV-DNA和HBeAg检测有显著相关性和互补性;前S1抗原可作为检测HBV复制新的血清标志物。更多还原

【Abstract】 Objective To study the clinical significance of HBV preS1-Ag,HBV-DNA and HBeAg in main groups of HBV infection.Methods HBV preS1-Ag and hepatitis B serum markers individually were detected by ELISA,and HBV-DNA by fluorescence quantitative PCR, then the detection results of the 308 cases were analysed.Results The serum markers of the 308 cases infected by HBV presented four modes which were "HBsAg,HBeAg,HBcAb","HBsAg,HBeAb,HBcAb","HBsAg,HBeAg","HBsAg,HBcAb".The total positive rate of preS1-Ag was 62.34%(192/308),and HBV-DNA was 78.90%(243/308).Compared HBeAg(+) groups with HBeAg(-)/anti-HBe(+) groups, the corresponding positive rates of preS1-Ag were 71.65%(139/194) and 46.49%(53/114),respectively.The difference of the two groups was significant (χ~2=18.30;P<0.01). Compared HBV-DNA(+) groups with HBV-DNA(-) groups,the corresponding positive rate of preS1 were 74.90%(182/243) and 15.38%(10/65), thus they had statistical differences(χ~2=74.85; P<0.01).The detection concordance of preS1-Ag and HBeAg was 64.94%,while the concordance of preS1-Ag and HBV-DNA was 76.95%.Conclusion The positive rate of HBV preS1-Ag detection in HBeAg(+)groups is higher than others.Obvious correlation and complementation are found among preS1-Ag,HBV-DNA and HBeAg,so it is concluded that preS1-Ag could be a new serum marker for detecting the copy of HBV.更多还原