【摘要】 目的了解人工肝治疗前、后重型乙型肝炎患者血清IL-18、总胆红素、γ-球蛋白水平和病毒标志物的变化,分析人工肝技术清除病理性有害物质效果。方法20例慢性重型乙型肝炎患者为人工肝治疗组(人工肝+内科综合治疗),血清IL-18用ELISA法检测、血清总胆红素用BeckmanCX4生化仪检测、血清γ-球蛋白分析用Sebia电泳仪,血清HBVDNA用实时荧光定量PCR法;对照组为20例仅进行内科综合治疗的慢性重型乙型肝炎患者。结果人工肝治疗组治疗前、后血清IL-18含量分别为(501·22±211·35)pg/ml和(302·56±87·33)pg/ml,治疗前、后血清IL-18均值经t检验差异有统计学意义(t=3·302,P<0·05);对照组治疗前、后血清IL-18含量分别为(485·36±178·21)pg/ml和(381·25±152·65)pg/ml,差异无统计学意义(t=1·90,P>0·05);人工肝治疗组治疗前、后HBVDNA定量结果分别为5·70×107±1·48×107和5·60×107±1·50×107,差异无统计学意义(t=0·44,P>0·05);治疗前、后血清总胆红素均值分别为(562·33±16·25)μmol/L和(302·99±63·54)μmol/L,差异有统计学意义(t=1·98,P<0·05);治疗前、后血清γ-球蛋白均值分别为(24·01±2·05)g/L和(21·98±2·26)g/L,差异无统计学意义(t=1·69,P>0·05)。结论人工肝技术可有效清除重型肝炎患者体内IL-18、总胆红素等病理性物质,可提高重型肝炎患者的存活率,为肝移植治疗争取时间,但对血清病毒标志物没有明显的清除作用。更多还原

【Abstract】 Objective To evaluate clarity function of pathological material with artificial liver technique by detecting sera IL₁8, total bilirubin（TBIL）, γ_globulin consistency and viral marker before and after the treatment. Methods Levels of sera IL₁8, TBIL, γ_globulin consistency and HBV DNA of 20 patients in artificial liver treatment group（artificial liver treatment and internal comprehensive therapy） were detected with ELISA, biochemistry of Beckman CX4 equipment, hydrgel protein of Sebia and real_time PCR. 20 patients of control group only with internal comprehensive therapy. Results In the artificial liver therapy group, （501.22±211.35）pg/ml and（302.56±87.33）pg/ml of sera IL₁8 were detected both before and after treatment, and there was a significance between the two times （t = 3.302,P<0.05）; in the control group, （485.36±178.21）pg/ml and （381.25±152.65） pg/ml of sera IL₁8 were detected both before and after treatment , and there was no difference between the two times （t=1.90,P>0.05）. In the artificial liver therapy group, the HBV DNA quantitative results before and after treatment were （5.70×10⁷± 1.48×10⁷ ）copies/ml and （5.60×10⁷±1.50×10⁷）copies/ml, they have no difference（t= 0.44, P>0.05）; the sera TBIL levels before and after treatment were（562.33±16.25） μmol/L and （302.99±63.54） μmol/L（t= 1.98, P<0.05）; γ_globulin consistency before and after treatment were（24.01±2.05）g/L and （21.98±2.26）g/L respectly （t= 1.69, P>0.05）. Conclusion The artificial liver technique can effectively clarity the pathological materials and is available to improve the survival rates and acquire precious time to liver transplantation for chronic server hepatitis B patients, but has no affect for viral marker.更多还原