【摘要】 目的应用组织芯片技术探讨人类肺癌中EphB4、HIF-1α和IGF-Ⅱ的表达情况以及三者表达的生物学意义。方法构建一个包含54例肺癌和10例正常肺组织的组织芯片蜡块,应用免疫组化SP法检测其EphB4、HIF-1α和IGF-Ⅱ蛋白的表达情况。结果病例组中EphB4、HIF-1α和IGF-Ⅱ蛋白的阳性率分别是44.4%、46.3%和42.6%,显著高于正常对照组(P<0.05);这三种蛋白的表达与肿瘤的大体类型、分化程度和TNM分期密切相关(P<0.05),而与患者性别、年龄、有无淋巴结转移无关(P>0.05)。三种蛋白的表达彼此之间存在显著正相关关系(P<0.01)。结论EphB4、HIF-1α和IGF-Ⅱ蛋白的表达可能与肿瘤的发生和恶性行为有关,三者之间存在着相互作用。更多还原

【Abstract】 Objective To investigate the relationship among the expression of EphB4,HIF-1αand IGF-Ⅱ in lung cancer and its biological significances.Methods Constructing a block with 54 lung cancer and 10 normal lung tissue on it.The expression of EphB4,HIF-1α and IGF-Ⅱ were detected in these specimens by using streptavidin-peroxidase(SP) immunohistochemical method,and 10 normal lung tissues as control.Results Among the 54 cases of lung cancer,the positive rate of EphB4 was(44.4%);(46.3%) of the tumors were positive for HIF-1α and(42.6%) were positive for IGF-Ⅱ.These three kinds of proteins expression were related to gross types,differentiations and clinical stages(P<(0.05)),but not to histological classification,age,sex and lymphoid metastasis(P>(0.05)).A highly positive correlation was observed between EphB4,HIF-1αand IGF-Ⅱ each other(P<(0.01)).Conclusions Overexpression of EphB4,HIF-1α and IGF-Ⅱmay play an important role in the pathogenesis,progression and malignant degree of lung cancer.There are interaction between them each other.更多还原