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阿克拉霉素A与DNA作用的光谱学研究

Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method

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【Author】 HU Min~ 1,2 , ZHANG Zhen-xi~ 1 , SHEN Guo-li~3, LIU Ya-li~3 1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, Xi’an Jiaotong University, Xi’an 710049, China 2. Department of Applied Chemistry, School of Science, Xi’an Jiaotong University, Xi′an 710049, China 3. State Key Laboratory for ChemoBiosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

【机构】西安交通大学生物医学信息工程教育部重点实验室；湖南大学化学化工学院；湖南大学化学化工学院生物医学工程研究所；陕西西安710049；西安交通大学理学院应用化学系；生物医学工程研究所；化学生物传感器与计量学国家重点实验室；湖南长沙410082；

【摘要】文章研究了阿克拉霉素A(ACR)吸收光谱和荧光光谱特性及其与DNA的相互作用。研究结果表明,ACR荧光光谱和吸收光谱受pH值影响较大,且随DNA加入产生减色效应。通过计算,ACR与DNA的结合常数比单纯以嵌入式与DNA结合的蒽醌类化合物大约1~2个数量级为2.7×106mol.L-1,结合位点数为0.67碱基对,ACR与DNA结合方式复杂,ACR为非经典嵌入剂。更多还原

【Abstract】 The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 ×10~6 mol·L~ -1 , and the binding site number is about 0.67 base pairs.更多还原

【关键词】阿克拉霉素A； DNA；荧光光谱；
【Key words】 Aclacinomycin； DNA； Fluorescence；

【基金】国家自然科学基金(20205005,20205004);西安交通大学自然科学基金资助项目

【文献出处】光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,2006年09期

【分类号】R96
【被引频次】15
【下载频次】240

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