【摘要】 利用胶体化学方法合成和表征了功能性L-半胱氨酸包覆的ZnS纳米粒子。在pH 5.12的NaAc-HAc水溶液介质中,当Δλ=190 nm时L-半胱氨酸包覆ZnS纳米粒子于268.0 nm处出现同步荧光峰。一定量蛋白质的加入能明显增强体系的荧光强度,并且峰强度增加值与蛋白质浓度间存在良好的线性关系,据此建立了一种高灵敏度的测定微量蛋白质的方法。用L-半胱氨酸包覆ZnS纳米粒子作为探针,不仅克服了有机染料可能出现的光漂白等缺点,而且本身不具毒性。将该法用于人血清试样中总蛋白的测定,其结果与临床数据一致。更多还原

【Abstract】 Nanometer-sized L-cysteine-capped ZnS particles as a fluorescence probe were synthesized,and the effect of proteins on the fluorescence intensity has been investigated.With Δλ=190 nm,the maximum and constant synchronous fluorescence（enhancement） was produced at 268.0 nm and pH 5.12 in the presence of proteins.A highly sensitive synchronous fluorescence method for the rapid determination of proteins was developed.Under optimum conditions,the calibration curve was linear over the range 0.05-6.0 μg·mL^-1 for human serum albumin（HSA）,and 0.05-8.0 μg·mL^-1 for γ-globulin（γ-G）,respectively.The relative standard deviations of seven measurements were 1.90% for 1.0 μg·mL^-1 HSA,and 1.65% for 1.0 μg mL^-1 γ-G.更多还原