【摘要】 文章以对巯基苯胺(PATP)作为标记分子,研究其在金电极上的吸附。发现对巯基苯胺分子是采用苯环直立于电极表面的吸附方式吸附于电极表面的。用于免疫检测时,对巯基苯胺分子先通过直立吸附的方法达到满单层吸附,然后通过胺基与抗体分子的羧基相连,形成一种复层结构,再与基底结合,得到固相抗体-抗原-标记抗体“三明治”结构。借助抗体上标记金纳米粒子的SERS信号达到单组分生物免疫检测的目的。更多还原

【Abstract】 The adsorption of p-aminothiophenol(PATP) molecule on the gold electrode was investigated.The result suggests that PATP was adsorbed perpendicularly onto the substrate.By immobilizing goat anti-mouse IgG on a glass slide modified by special organic molecules,the antibody/antigen/reporter-labeled immunogold nanoparticle sandwich assay was build for surface-enhanced Raman spectroscopy(SERS) measurements.The p-aminothiophenol(PATP) was used as SERS-reporter molecule.Thus the authors can identify the single type biospecific species by SERS signal of the reporter molecules which are coimmobilized with antibody molecules in gold colloids.更多还原