【摘要】 以溴化乙锭(EB)为荧光探针,结合紫外光谱法研究了壳聚糖、羧甲基壳聚糖、羧甲基纤维素与DNA的相互作用,药物分子与DNA作用程度可用作用常数D来表示,实验表明:几种生物多糖与DNA分子的作用强弱顺序为:壳聚糖>羧甲基壳聚糖>羧甲基纤维素;多糖主要以嵌插方式与DNA作用,使EB-DNA荧光猝灭。更多还原

【Abstract】 The interaction of chitosan(CTS),carboxymethyl chitosans(CMCTS),and carboxymethyl cellulose(CMCL) with DNA was investigated through ethidium bromide(EB) as a sensitive fluorescence probe,and ultraviolet absorption spectra.The constant D can directly denote the degree of interaction of polysaccharide with DNA.The results indicated that the interraction sequence of biological polysaccharides with DNA is as follows: CTS>CMCTS>CMCL.The polysaccharides were mainly bound to DNA by intercalated binding,which resulted in fluorescence quenching effect of EB-DNA.更多还原