【摘要】 4个不同渗透压水平(280、320、360、400 mOsm)的鸡精冷冻稀释液,以甘油(GL)、二甲基亚砜(DMSO)和N,N二甲基乙酰胺(DMA)分别作为抗冻剂进行了冷冻试验.结果表明,添加8%甘油、4%DMSO和11%DMA的冷冻稀释液,4个不同渗透压间冻后精子活率均有显著差异(P<0.05),而且精子活率随冷冻稀释液渗透压的升高而升高;添加5%和10%甘油的冷冻稀释液,渗透压对解冻精子活率影响不显著;在400 mOsm渗透压条件下,8%甘油组冷冻后精子活率高于其他抗冻剂组;3种抗冻剂4个渗透压水平冷冻,平衡时间长短(30 min和90 min)对解冻后精子活率没有影响.更多还原

【Abstract】 Glycerol,DMSO and DMA were supplied in diluents with four different osmotic pressures(280,320,360,400 mOsms)as cryoprotector to frozen chicken’s semen.The results showed that: there were significant difference of frozen-thawed motility in each osmotic pressure suplied with 8 %GL,4 %DMSO and 11 %DMA in diluents(P<0.05),and the motility was promoted with the rise of osmotic pressure;while in diluents with 5 % or 10 % glycerol had no significant difference.In this study,the diluents of 400 mOsm gained the highest sperm frozen-thaw motility in three cryoprotector and three glycerol proportion.Among three cryoprotectors only 8 % GL achieved high motility.Under four different osmotic pressures,there was no significant difference between 30 mins and 90 mins to motility(P>0.05).更多还原