【摘要】 主要介绍了利用NCBI、DDBJ、Blockmaker、CodeHop和Oligo6.0等数据库和生物软件进行简并引物设计的方法。利用设计的6对简并引物进行RT-PCR,从荔枝(LitchichinensisSonn.)果实中克隆到2个长度分别为510bp和582bp的cDNA片段。通过Blastx检索Genbank进行同源性比对后,发现2片段都与其它植物的HMGR基因具有较高的氨基酸序列同源性。研究表明,该程序化设计的简并引物可信性强,阳性率高,能迅速得到满意结果。更多还原

【Abstract】 This paper aims to introduce the method of designing degenerate primers by using some internet databases and biological softwares such as NCBI, DDBJ, Blockmaker, CodeHop and Oligo 6.0. Using the designed 6 pairs of primers to carry out RT-PCR, 2 cDNA fragments with the length of 510 bp and 582 bp respectively were cloned from the litchi(Litchi chinensis Sonn.)young fruit. These 2 fragments were compared in the Genbank by blastx, and the results indicated that the 2 fragments had considerable identities with the HMGR genes of other plants. It is considered that the degenerate primers obtained from this programming design are practicable, and also get satisfied results and high positive rates.更多还原