【摘要】 通过对Botriosphaeria dothidea分生孢子在6种非生物表面及苹果果面上的吸附作用的研究,发现在各种非生物表面上分生孢子表现出不同的吸附特性。封口石蜡膜、载玻片和凡士林包衣载玻片易于吸附孢子,其中石蜡膜最易被孢子吸附,而聚丙烯带和石蜡包衣载玻片不易被孢子吸附。在石蜡膜上滴加孢子后20 min即可达最大吸附,而在载物玻片,塑料膜,石蜡包衣载物玻片,凡士林包衣载物玻片和聚丙烯膜上需40～60 min才能达到最大吸附。在果实皮孔处,孢子吸附随孢子浓度及吸附时间增加而增加。孢子浓度在2．0×104-5．0×104／mL范围内,孢子在皮孔处的吸附数量无显著差异。但是在非皮孔处,孢子浓度至少需4×104／mL而且需吸附20～30 min后才能达到最大吸附。伴刀豆蛋白A(Conncavaline A)是孢子吸附的一种高效抑制剂,甚至在100μg/mL的浓度即足以抑制孢子吸附。更多还原

【Abstract】 The attachment characteristics of Botriosphaeria dothidea conidiospores on the surfaces of 6 abiotic objects and apple skin were studied. The spores showed various behaviors in surface attachment on different kinds of abiotic objects. Spores were easily attached to parafilm, glass slide and vasiline-coated slide, especially to parafilm, but not so easily attached to polypropylene tape and paraffin-coated slide. The maximum attachment was generally realized about 40 to 60 min after deposition of spores on the surfaces of tested objects except on parafilm where the maximum attachment was at 20 min after spore deposition. On apple lenticel, the spore attached increased sharply when the spore concentration was above 5.0×104 spores/mL. From 2.0×104 to 5.0×104 spores/mL, there was no difference in the number of conidiospores attached on the lenticel. On the non-lenticel surface, spore attachment reached the maximum 20 to 30 min after spore deposition with a spore concentration no less than 4.0×104 spores /mL. ConA was a highly effective inhibitor of spore attachment. It effectively blocked spore attachment on apple surface at 100μg /mL.更多还原