【摘要】 利用AFLP分析鉴定了7个柑橘变异类型,从64对引物组合中筛选了8对引物组合,共得到334个扩增位点2099条带数据。7个变异类型与亲本品种之间多态性带为36至92条,变异类型的多态性带数与亲本品种总带数的比例为0.1721至0.4182。说明变异类型的遗传基础已经发生了不同程度的改变,7个柑橘变异类型应是芽变,具备了成为新品种的遗传基础,特别是红皮冰糖脐橙选育成功将填补高糖脐橙空白。研究同时表明,AFLP具有扩增谱带多,谱带清晰,扩增信息量大的特点。根据谱带位点差异即可鉴定柑橘变异是否遗传,多态性位点数也反映了性状变异程度。AFLP方法1次可同时鉴定多个变异类型,是柑橘新品种选育早期鉴定灵敏、可靠、有效的方法。更多还原

【Abstract】 AFLP technique was used to determine DNA polymorphism of citrus mutants originated from 2 different parental cultivars in Hunan province. 8 was selected out of 64 pairs of primer combinations and 2099 polymorphism bands were amplified. There were 36 to 92 polymorphism bands between mutants and parental cultivars. Ratio of polymorphism bands of every mutant to total bands of parental cultivars was 0.1721 to 0.4182. The results showed that 7 different type citrus mutants are bud mutant, which has got genetic basis to be selected as new cultivar. Experiment proved that AFLP is characterized with abundant, clear polymorphism bands and is very effective analysis method for identification of citrus mutants.更多还原