【摘要】 用紫外光谱法和荧光光谱法研究了氢氯噻嗪(HCT)和小牛胸腺DNA(ctDNA)之间的相互作用。在pH7.0的磷酸盐缓冲溶液中,HCT的荧光激发峰和发射峰分别位于278nm和360nm处。ctDNA的加入对HCT的荧光存在着很强的荧光猝灭作用,这种荧光静态猝灭作用是由ctDNA和HCT键合引起,键合常数为1.12×10-4L/mol(25℃)。采用紫外光谱,离子强度的影响和I-猝灭等条件实验研究了HCT与DNA间的相互作用。DNA浓度的变化不改变它们的作用,属于沟槽作用模式。更多还原

【Abstract】 The interactions of Hydrochlorothiazide (HCT) with native and denatural calf thymus DNA were studied by UV and fluorescence spectrometry.In the case of 0.1mol/L PBS buffer solution(pH 7.0),the complex formation results in the red shift and intensity decrease in absorption and excitation spectra of fluorescence with the formation constants of 1.12×10 -4 L/mol.The interaction between Hydrochlorothiazide and DNA is independent of the concentration of DNA,belonging to electrostatic binding model.更多还原