【摘要】 目的:研究三七总皂苷(total sapon ins of panax notoginseng,PNS)对异丙肾上腺素所致大鼠心肌肥厚的保护作用。方法:用异丙肾上腺素(isoproterenol,ISO)5 mg.kg-1.d-1,sc,连续7 d,建立大鼠心肌肥厚模型。造模第二天开始给大鼠腹腔注射PNS 25和50 mg.kg-1.d-1,连续14 d,测定全心重量指数(HW/BW)、左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量,及Na+-K+-ATP酶和Ca2+-ATP酶活性;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果:ISO模型组大鼠的HW/BW、LVI明显升高;左心室Hyp、AngⅡ含量增高,NO水平下降,Na+-K+-ATP酶和Ca2+-ATP酶活性降低。PNS能明显提高心肌组织NO水平及Na+-K+-ATP酶和Ca2+-ATP酶活性,降低胶原的含量,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚。结论:PNS对ISO所致大鼠心肌肥厚具有保护作用。更多还原

【Abstract】 Objective: To investigate the protective effects of total saponins of panax notoginseng（PNS） on myocardial hypertrophy induced by isoproterenol（ISO） in rats.Methods: Myocardial hypertrophy model of rats were induced by injection of ISO(5mg·kg^-1·d^-1,sc) for 7d,from day 2,the rat were administered with PNS 25 and 50mg·kg^-1·d^-1,ip for 14d,the control and ISO model group were received saline injection.Then, the heart-weight（HW）,left ventricular weight（LVW）,the ratio of HW/BW and LVW/BW（LVI） were measured;the content of hydroxyproline and angiotensin（AngII） ,the level of nitric oxide（NO） and the activity of Na⁺-K⁺-ATPase Ca²⁺-ATPase in left ventricle were determined by spectrophotometry and radioimmunoassay,respectively.Results: In ISO model group,HW/BW、LVI and the content of hydroxyproline and AngII in legt ventricle markedly increased;the level of NO and the activity of Na⁺-K⁺-ATPase,Ca²⁺-ATPase in the left ventricle were decreased.Treatment with PNS significantly reduced the HW/BW,LVI,decreased the AngII and the hydroxyproline content,increased the level of NO and the activity of Na⁺-K⁺-ATPase Ca²⁺-ATPase.Conclusion: PNS was shown to prevent remodeling of myocardial hypertrophy induced by ISO in rats.更多还原