【摘要】 由于细胞分化不完全及表型不稳定,目前仍没有非常适合代替原代胰岛β细胞进行功能研究用的细胞系。已经建立的胰岛细胞系主要分两类:动物胰岛β细胞瘤起源的细胞系(如RIN、INS细胞系)和人β细胞起源的细胞系,各种细胞系的生物学特征方面各不相同,但都保留了某些正常胰岛细胞功能特征。采用基因工程的方法和挑选更优越的亚系是目前改善细胞系功能的主要手段。更多还原

【Abstract】 Recently,there are not suitable islet-derived cell lines which can take the place of the primary cells for functional studies yet,because their phenotypic features are undifferentiated or unstable.The established cell lines are mainly divided into two categories: cell lines from animal insulinoma cells(such as RIN line,INS line)and human β-cell derived lines.Each cell line has different biological characteristics,but they all retain many key functional attributes of normal islets.Genetic engineering and isolation of more suitable subclones are two different approaches for functional improvements of preexisting cell lines.更多还原