【摘要】 目的建立一种灵敏度较高的高效液相色谱法,测定大鼠血浆中抗肿瘤化合物SYUIQ-5的浓度。方法流动相用35mmol·L-1磷酸氢二钾缓冲液(pH8.0)-甲醇-三乙胺(30∶70∶0.05),血浆样本用乙醚萃取,紫外检测波长为278nm。结果线性范围为30~1500μg·L-1,线性关系良好,最低检测限为20μg·L-1,日内RSD≤5.2%,日间RSD≤3.8%;提取回收率为72.1%~80.5%(n=5),相对回收率为96.8%~105.1%。结论本方法灵敏度高,重现性好,精密度和准确性好,适于SYUIQ-5的药代动力学研究。更多还原

【Abstract】 Objective To determinate the plasma concentration SYUIQ-5 by establishing HPLC method,a novel anti-tumor compund in rat. Methods Plasma samples were cleaned up by extraction with diethyl ether. The chromatogram was monitored by UV detection at a wavelength of 278 nm. The mobile phase consisted of 35 mmol·L~ -1 dipotassium hydrogen phosphate (pH 8.0)-methanol -triethylamine(30∶70∶0.05).Results A linear range was 30~1 500 μg·L~ -1 , the lower limit of detection was 20 μg·L~ -1 . The intra- and inter-run precision were lower than 5.2% and 3.8 %(n=5), respectively. The extraction recovery was 72.1% ~80.5% (n=5) and the method recovery was 96.8%~105.1%.Conclusion The me- thod is shown to be accurate and repetitive, simple to operate, suitable for clinical pharmacokinetic study .更多还原