【摘要】 培养30 d的菘蓝无菌幼苗经250 mmol/L NaCl、自然干旱、20 mmol/L ABA、4℃低温等不同方式逆境处理,不同时间内取样,用高效液相色谱法对其中靛玉红的含量进行测定。结果表明:盐、干旱、ABA处理12 h内能提高菘蓝幼苗中靛玉红含量59.05%~161.67%,低温胁迫处理24 h也能提高靛玉红含量141.4%,说明适当逆境胁迫处理能有效提高菘蓝幼苗体内靛玉红的累积量,其中250 mmol/L NaCl胁迫处理效果最为显著。更多还原

【Abstract】 The seedlings of Isatis indigotica Fort.cultivated for 30 days in aseptic environment were treated with 250 mmol/LNaCl,natural drought method,20 mmol/L ABA,4℃ low temperature,respectively.The content of indirubin was extracted from Isatis indigotica Fort.seedlings at the indicated times and analyzed using HPLC method,the results showed that the content of indirubin could increased up to 59.05%～161.67% in salt,drought and ABA treatment during 12 h.The content of indirubin increased up to 141.4% under low temperature stress for 24 h.These implied that the content of indirubin in the Isatis indigotica Fort.seedlings could accumulate by appropriate stress and ABA treatment efficiently.The 250 mmol/L NaCl stress treatment had the most effect.更多还原