【摘要】 目的研究β-cat在新生儿细支气管中的表达情况,探讨β-cat在新生儿支气管发育中的作用。方法采用免疫组化(SP)法,检测22例尸检新生儿β-cat、GSK-3β在细支气管上皮细胞的表达,以12例儿童为对照。结果β-cat在新生儿组有6例出现细胞膜表达降低、3例出现胞质表达、2例胞核表达;对照组中2例胞膜表达减少,1例胞质表达。β-cat的平均光密度值在新生儿组为0·112±0·024,对照组为0·128±0·037。两组比较P>0·05,无显著性差异;GSK-3β平均光密度值在对照组为0·147±0·037,新生儿组为0·115±0·028,两组比较P<0·05,有显著性差异。结论β-cat在新生儿细支气管的异位表达,提示Wnt信号与支气管的发育成熟有关。更多还原

【Abstract】 Objective To study the expression of beta-catenin (β-cat) and glycogen synthase kinase-3β(GSK-3β) on the bronchiolar epithelia of the newborn, and to evaluate the role of β-cat in the bronchiole development.Methods The expression of β-cat and GSK-3β were detected in the bronchiolar epithelia of 22 autopsy newborns and 12 children without the bronchiolar disease. Results The cell membrane expression of β-cat in 6 cases of the newborn group was lower than that in the control group, but the expression in the cytoplasm and nuclei of the bronchiolar epithelial cells was detected in the newborns. The expression of GSK-3β in the cytoplasm was significantly lower in the newborn group than in the control group. Conclusion The varied expressions in membrane, cytoplasm and nucleus of the bronchiolar epithelium in the newborns may be related with the active signal of the Wnt pathway in the bronchiole development.更多还原