【摘要】 目的观察正常成人真皮内巨噬细胞和树突状细胞的分布、形态学和密度。方法正常人8例,取面部、躯干、四肢近端、四肢远端、手掌和足跖6个部位皮肤,进行纵行与水平切片。CD1a、CD68、OKM5单克隆抗体和FⅩⅢa多克隆抗体染色。结果CD1a和FXⅢa阳性细胞多位于皮肤附属器和血管周围。CD68阳性细胞在真皮浅层呈网状分布,真皮深层密度明显降低。各个部位的OKM5阳性细胞数较少。真皮内各个部位CD68阳性细胞高于相同部位CD1a、FⅩⅢa和OKM5阳性细胞(P<0·0001)。结论在真皮内存在大量的呈网状分布的CD68阳性的单核-巨噬细胞;同时在真皮,特别是浅层,有较多的树突状细胞,如郎格汉斯细胞和真皮树突状细胞,但其数量远低于CD68阳性的单核-巨噬细胞。CD68阳性的单核-巨噬细胞和真皮内的树突状细胞,是真皮内的主要防御细胞。更多还原

【Abstract】 Objective To observe the distribution, morphology and density of macrophages and dendritic cells in the normal adult human dermis. Methods Normal adult skin from 6 locations (face, trunk, proximal limbs, distal limbs, palms and soles) was collected for the study, The horizontal and longitudinal sections of the skin were stained with ABC procedure with anti CDla, anti-CD68 and anti-OKM5 monclonal antibodies or anti-FXⅢa poly-antibody. Results The CD1a and FXⅢa-positive cells mainly located the skin appendages and arounded the vessels. In the superficial dermis CD68 positive monocytes/macrophages form a dense network. This network of CD68 positive cells continued to and surround the blood vessels and skin appendages. Lower densities of CD68 positive dendritic cells were found in the deep (reticular) dermis, dispersed between collagen bundles. In all locations the densities of OKM5 positive cells were low. In normal human skin, the numbers of dermal CD68 positive monocytes/macrophages were significant greater than FⅩⅢa, OKM5 and CD1a positive cells. Conclusion CD68 monocytes/macrophages are the predominant cells in the dermis, forming a network from superficial to deep dermis. There are significant numbers of dendritic cells such as Langerhans cells and dermal dendritic cells, especially in the superficial dermis. These are the main cell types in the dermis in an organized fashion for the defense of the skin against external invasion.更多还原