【摘要】 目的探讨近年用来治疗恶性肿瘤的视黄酸与细胞因子(TNF-α、IL-1β、IL-4、IL-6、IFN-γ)对人肺癌细胞株A549分泌C3及Β因子的影响。方法分别用ELISA、Western blot和RT-PCR检测培养上清中C3及B因子的水平及细胞C3及B因子mRNA表达。结果TNF-α、IL-1β、IL-4、IL-6和IFN-γ诱导A549细胞分泌C3及B因子是未处理组的6.84、11.0,7.27、8.04,6.16、1.08,4.75、1.43和2.10、1.59倍。视黄酸对Α549细胞分泌C3及B因子没有影响,但它能增强TNF-α、IL-1β、IL-4、IL-6和IFN-γ诱导的A549分泌C3及B因子分别是TNF-α、IL-1β、IL-4、IL-6和IFN-γ组的1.46、1.90,1.44、1.90,1.18、1.69,0.94、2.52,和1.09、2.39倍。RT-PCR半定量分析C3及B因子mRNA的结果与蛋白水平相似。结论视黄酸能调节诸细胞因子增强诱导人肺癌细胞株A549细胞分泌C3及B因子,这可提高细胞因子对肿瘤细胞的杀伤作用。更多还原

【Abstract】 Objective To explore the effects of retinoic acid(RA) and cytokines(TNF-α,IL-1β,IL-4,IL-6 and IFN-γ),which have been used in the treatment of malignant tumor in recent years,on C3 and factor B secretion in human lung cancer cell line A549.Methods The levels of C3 and factor B in culture supernatant were detected by ELISA(enzyme-linked immunosorbant assay) and Western blotting,and RT-PCR(reverse transcription polymerase chain reaction) was used to examine the mRNA expression of C3 and factor B.Results Cytokines induced the secretion of C3 and factor B.The levels of C3 induced by TNF-α,IL-1β,IL-4,IL-6 and IFN-γ were 6.84,7.27,6.16,4.75 and 2.10 times respectively of those in control groups,whereas those of factor B were 11.0,8.04,1.08,1.43 and 1.59 times.While RA alone had no effect on the secretion of C3 and factor B,it enhanced the secretion of C3 and factor B induced by cytokines.The levels of C3 in groups that combined RA respectively with TNF-α,IL-1β,IL-4,IL-6 and IFN-γ were 1.46,1.44,1.18,0.94 and 1.09 times of those in TNF-α,IL-1β,IL-4,IL-6 and IFN-γ groups separately,and those of factor B were 1.90,1.90,1.69,2.52 and 2.39 times.Likewise,mRNA expressions of C3 and factor B tested by RT-PCR were up-regulated by cytokines and RA in the A549 cells.Conclusion Cytokines and RA induce and enhance the secretion of C3 and factor B in human lung cancer cell line A549.Thus they regulate the complement system and play an important role in the management of malignant tumors.更多还原