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蔗渣半纤维素不同组分的水解
Hydrolysis of Different Hemicelluloses Extracted from Bagasse
【摘要】 对蔗渣半纤维素不同组分用酸或碱水解、酶解、酶解后再酸/碱水解的不同方法进行水解,利用高效液相色谱(HPLC)分析水解液中单糖的组成,探讨半纤维素不同组分的水解过程与产物组成的关系。实验结果显示对半纤维素A用半纤维素酶(酶活400 IU/g)法水解比用20%NaOH水解效果好,木糖得率达34.87%;而对于半纤维素B,采用酶法水解比7%H2SO4水解得率相差较大,采用酶解后7%H2SO4水解可得得率为23.60%。
【Abstract】 Different hemicelluloses extracted from bagasse were hydrolyzed by acid or alkali hydrolysis,enzymolysis,enzymolysis after acid or alkali hydrolysis.The compositions of monose in hydrolyzate were analyzed by HPLC,and the relation between different hydrolyzing conditions and the composition of the products was set up.The results of experiment showed that to hemicellulose A,the enzymolysis with hemicellulase(enzymatic activity 400IU/g) got a higher productivity than alkali hydrolysis,the yield of xylose was 34.87%,but to hemicellulose B,the productivity of enzymolysis was lower than that of acid hydrolysis,the yield of xylose after hemicellulase-acid hydrolysis was 23.60%.
【Key words】 bagasse; hemicellulose; hemicellulase; hydrolysis; HPLC;
- 【文献出处】 造纸科学与技术 ,Paper Science & Technology , 编辑部邮箱 ,2006年04期
- 【分类号】TS711
- 【被引频次】9
- 【下载频次】688