【摘要】 目的:探讨放疗对喉癌患者T淋巴细胞(T-LC)亚群、白细胞介素-2(IL-2)、肿瘤坏死因子(TNF)及B cl-2表达量的影响。方法:对40例喉癌患者血CD 4、CD 8、IL-2、TNF及肿瘤组织中B cl-2采用单克隆抗体碱性磷酸酶法、EL ISA放免法和免疫组化法进行检测,并与30例正常人作对照。结果:喉癌患者血CD 4、IL-2及TNF含量均明显低于正常者(P<0.01),放疗后CD 4、IL-2及TNF含量低于放疗前;喉癌患者放疗前血CD 8含量与正常人比较差异无显著性(P>0.05),放疗后则明显升高(P<0.05);放疗后喉癌组织中B cl-2表达水平低于放疗前(P<0.05)。结论:检测喉癌患者T-LC亚群、IL-2及TNF,对评价患者免疫状况及活体病变程度、判断疗效有指导意义;B cl-2则对判断肿瘤细胞存活状况有指导意义。更多还原

【Abstract】 Objective: To investigate the effect of radiotherapy on the expression of T-lymphocyte subpopulations,interleukin-2(IL-2),tumor necrosis factor(TNF) and Bcl-2 in laryngeal cancer patients.Methods: The content of T-lymphocyte subpopulations,IL-2,CD4,CD8,TNF in blood and Bcl-2 in cancer tissue of 40 laryngeal cancer patients were detected by clone antibodies,alkialine phospalase method,ELISA,radia immunity and immunohistochemial technigue,respectively,with 30 cases of normal people as the control.Results: The blood content of CD4,IL-2 and TNF in laryngeal cancer patients were lower than in healthy people(P<0.01),and in pre-radiotherapy patients were higher than in post-radiotherapy patients. Although there was no difference of CD8 content between in pre-radiotherapy patients and in healthy people(P>0.05),CD8 content increased after post-radiotherapy(P<0.05);the Bcl-2 expression in post-radiotherapy patient was lower than in pre-radiotherapy patients(P<0.05).Conclusion: Laryngeal cancer may erode the patient’s immuno-reactivity and the radiotherapy can exacerbate this process.更多还原