【摘要】 目的研究脐血单个核细胞体外多向诱导分化为LAK,CD3AK,CIK细胞及体外对胆囊癌细胞株GBC-SD杀瘤活性的变化。方法采用IL-2刺激诱生LAK细胞;用CD3单抗和IL-2刺激诱生扩增CD3AK细胞;加入IFN-γ,24h后加入IL-1,CD3单抗,IL-2刺激诱生CIK细胞,观察它们的扩增情况,并分析其相互关系;取培养的CIK细胞用流式细胞仪分析表型;用MTT法以胆囊癌细胞株GBC-SD为靶细胞,分组测定LAK,CD3AK,CIK细胞的杀伤活性。结果CD3AK和CIK细胞的扩增能力远大于LAK细胞(P<0.05)。CIK细胞和CD3AK相比,在前期无明显差异,至第20天左右时CIK细胞的扩增倍数显著高于CD3AK(P<0.05)。流式细胞仪分析结果显示CIK细胞培养后,其主要效应细胞CD3+CD56+细胞较培养前显著增加(P<0.05),CD8+细胞也较培养前显著增加(P<0.05)。CD3AK和CIK细胞对胆囊癌细胞株GBC-SD的杀伤活性均显著高于LAK细胞(P<0.05),而CIK细胞杀伤活性又强于CD3AK细胞(P<0.05)。结论CIK细胞具有更高的扩增能力和更强的杀伤活性,是肿瘤过继免疫治疗中更为有效的杀伤效应细胞。更多还原

【Abstract】 Objective To study multi-direction derivation from cord blood mononuclear cells to CD₃AK, LAK and CIK cells as well as variations of killing activity to the GBC-SD gallbladder cancer cell strain in vitro.Methods CD₃mAb and IL-2 were used to induce CD₃AK cells;IL-2 was used to induce LAK cells; IFN-γ was used in the beginning, then IL-1, CD₃mAb and IL-2 were used to induce CIK cells after 24 h for observing amplification status and analysis of the relationship; cultivated CIK cells were taken to analyze phenotype by flow cytometer, subsequently, the GBC-SD gallbladder cancer cell strain was taken as target cells to evaluating killing activity of CD₃AK, LAK and CIK cells, respectively by MTT method.Results The amplification activity of CD₃AK and CIK cells were all far higher than LAK cells （P<0.05）. Compared with CD₃AK cells, the amplification activity of CIK cells had no obviously difference at prophase, but was far higher than CD₃AK cells at about 20 d （P<0.05）. The analytic results of flow cytometer disclosed that the amount of CD3+ CD 56 + cells which were major effector cells after CIK cells being cultivated was far higher than before （P<0.05）, moreover, the amount of CD₈+ cells was far higher than before as well （P<0.05）. The killing activities of CD₃AK and CIK cells to the GBC-SD gallbladder cancer cell strain were all far higher than LAK cells, besides, killing activity of CIK cells was far higher than CD₃AK cells （P<0.05）.Conclusion CIK cells have higher amplification activity and higher killing activity,which can be taken as more effective killing cells applied to the tumor adoptive immunotherapy.更多还原